Anti-Inflammatory Effect Of Grape Seed Extract On Bronchial Asthma
Conference: 5th International Conference on Quality and Evidence Based in Prophetic Medicine
Keywords: Prophetic Medicine, Quality
Abstract
Bronchial asthma remains a growing major public health issue worldwide. While anti-inflammatory agents are commonly used for treatment, natural remedies like grape seed extract (GSE), known for its potent antioxidant and anti-inflammatory properties, may offer therapeutic potential.
This study aimed to evaluate the effects of GSE on allergic airway inflammation in a mouse model of asthma. Airway inflammation was induced via intraperitoneal injection of ovalbumin (OVA) plus aluminum hydroxide. Fifty male mice were divided into five groups:
- Negative control (PBS),
- Positive control (OVA-asthma model),
- Asthma mice treated with montelukast (30 mg/kg),
- Asthma mice treated with GSE (150 mg/kg), and
- Asthma mice treated with both GSE and montelukast.
Treatments were administered on days 27–30, with montelukast and GSE given one hour before each challenge. Inflammatory cytokines IL-5 and IL-13 were measured in bronchoalveolar lavage fluid (BALF), and plasma immunoglobulin E (IgE) levels were determined. Lung levels of malondialdehyde (MDA), nitric oxide (NO), and superoxide dismutase (SOD) were assessed, and lung tissues were examined histologically.
Results showed a significant increase (p<0.001) in IL-5, IL-13, IgE, NO, and MDA levels, and a decrease in SOD in the asthma group compared to controls. Histopathological examination confirmed inflammation with bronchiolar and perivascular infiltrates. Treatment with GSE, alone or combined with montelukast, significantly improved all measured parameters. Notably, the combined treatment offered superior benefits compared to montelukast alone.
These findings suggest that GSE, especially when used alongside montelukast, enhances immune regulation and may reduce adverse effects, supporting its potential as a novel anti-inflammatory natural remedy for asthma treatment.