Emirates Scholar Research Center - Research Publishing & Indexing Center

From Birds To Human, The Common Strategies Towards One Health

Author(s): Yahia CHEBLOUNE

Conference: Dubai One Health Conference

Publication Date: 23 June, 2023

Keywords: RSV, CAEV, SIV, HIV, SHIV, Vaccines


Infectious Pathogens Like Retroviruses Can Cause Variety Of Degenerative Multisystemic Lethal Diseases In Human And Large Number Of Animal Species. Rous Sarcoma Virus (RSV) Was The First Described Transmissible Retrovirus Causing Solid Lethal Tumors In Infected Chickens. The Classical Strategies Of Vaccine Development Failed To Produce An Effective Vaccine That Can Protect Birds From Infection Or RSV-Associated Disease. We Used The Innovative Molecular Biology Tools To Engineer A Replication-Defective Vector Expressing The Env Glycoproteins Of RSV. We Used This Vector To Transduce Primary Chicken Fibroblasts And Express RSV Glycoproteins At Their Cell Surface. Cell Lysates Of These Cells Were Used As Cell-Associated Antigen To Immunize Adult Chickens. All Immunized Chickens Produced High Titers Of Protective Antibodies Against The Highly Pathogenic RSV Strain (1). This Technology Was Transposed To New Castle Disease Virus In Chicken Chowing Similar Protection Results Against NDV Pathogenic Strain (2). To Increase The Safety And The Specificity We Moved To Development Of DNA Vaccines In Mammals Against Lentiviruses. As Lentiviruses Are Chronic Infectious Disease That Cause Persistent Progressive Degenerative Diseases, Viruses Use Multiple Strategies To Persist Despite Host Specific Immune Responses. These Strategies Rendered Difficult Development Of Effective Vaccines. We Developed Innovative LentiDNA Vectors As Vaccines (3-7) And Used Them In Animal Models To Demonstrate Their Efficacy To Induce Long Lasting Virus-Specific Immune Responses And Protection Against Pathogenic Viruses. Our Data Showed That Our LentiDNA Vaccines Are Powerful Inducers Of Long-Lasting Immune Responses. These Immune Responses Correlated With Protection Against Homologous And Heterologous Pathogenic Virus Strains (8-10). Cited References. – Chebloune, Y., Rulka, J., Cosset, F.L., Valsesia, S., Ronfort, C., Legras, C., Drynda, A., Nigon, V.M. And Verdier, G. 1991. Immune Response And Resistance To Rous Sarcome Virus (RSV) Challenge Of Chickens Following Immunization With Cell Aassociated Env Glycoproteins With A Recombinant Avian Leukosis Virus. J. Virol. 65, 5374-5380. – Cosset, F.L., Bouquet, J.F., Drynda, A., Chebloune, Y., Reysonelonge A., Kohen, G., Nigon, V.M., Desmettre, P. And Verdier, G. 1991. Newcastle Disease Virus (NDV) Vaccine Based On Immunization With Avian Cells Expressing The NDV Hemagglutinin-Neuraminidase Glycoprotein. Virology 185, 862-866. – Beyer J. C., Y. Chebloune, L. Mselli-Lakhal, I. Hِ Tzel, N. Kumpula-McWhirter, And W. P. Cheevers 2001. Immunization With Plasmid DNA Expressing Caprine Arthritis-Encephalitis Virus Envelope Gene: Quantitative And Qualitative Aspects Of Antibody Response To Viral Surface Glycoprotein. Vaccine. 19: 1643-1651. – González B., Reina R., García I., Andrés S., Glaria I., Alzueta M., Mora M.I., Jugo B.M., Arrieta-Aguirre I., Pérez De La Lastra J.M., Rodríguez D., Rodríguez J.R., Esteban M., Grilló M.J., Blacklaws B.A., Harkiss G.D., Chebloune Y., Luján L., De Andrés D., And B. Amorena. 2005. Mucosal Immunization Of Sheep With A Maedi-Visna Virus (MVV) Env DNA Vaccine Protects Against Early MVV Productive Infection. Vaccine 23 (34): 4342-4352. – Hegde, R., Z. Liu, G. Mackay, M. Smith, Y. Chebloune, O. Narayan, And D. K. Singh. 2005. Antigen Expression Kinetics And Immune Responses Of Mice Immunized With Noninfectious Simian-Human Immunodeficiency Virus DNA. J. Virol. 79:14688-14697. – Chebloune Y, Moussa M, Arrode-Brusés G, Ronfort C, Bose D, Gagnon J, Gumber S, Villinger T, Byrareddy SN, Kozlowski PA, Gosse L, Dereuddre-Bosquet N, Le Grand R, Villinger F. 2020. A Single Lentivector DNA Based Immunization Contains A Late Heterologous SIVmac251 Mucosal Challenge Infection. Vaccine. 2020 May 6;38(21):3729-3739. Doi: 10.1016/J.Vaccine.2020.03.053. Epub 2020 Apr 9. PMID: 32278522 – Arrode-Brusés G, Sheffer D, Hegde R, Dhillon S, Liu Z, Villinger F, Narayan O, Chebloune Y. 2010. Characterization Of T-Cell Responses In Macaques Immunized With A Single Dose Of HIV DNA Vaccine. J Virol. 2010 Feb;84(3):1243-1253. – Leroy LA, Mac Donald A, Kandlur A, Bose D, Xiao P, Gagnon J, Villinger F, Chebloune Y.Vaccines (Basel). 2022 Cytokine Adjuvants IL-7 And IL-15 Improve Humoral Responses Of A SHIV LentiDNA Vaccine In Animal Models. Mar 17;10(3):461. Doi: 10.3390/Vaccines10030461.PMID: 35335093 – Moussa M, Arrode-Brusés G, Manoylov I, Malogolovkin A, Mompelat D, Ishimwe H, Smaoune A, Ouzrout B, Gagnon J, Chebloune Y. A Novel Non-Integrative Single-Cycle Chimeric HIV Lentivector DNA Vaccine. Vaccine. 2015 May 5;33(19):2273-82. Doi: 10.1016/J.Vaccine.2015.03.021. Epub 2015 Mar 28. PMID: 25825333 – Yankee TM, Sheffer D, Liu Z, Dhillon S, Jia F, Chebloune Y, Stephens EB, Narayan O. 2009. Longitudinal Study To Assess The Safety And Efficacy Of A Live-Attenuated SHIV Vaccine In Long Term Immunized Rhesus Macaques. Virology. 2009 Jan 5;383(1):103-11.

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