Biological Activity Of Sider Plant (Ziziphus Spina Christi L) Against Microbial Diseases And Its Essential Role Of Infection And Prevention Control

Conference: 4th International Conference In Prophetic Medicine Research

Keywords: Prophetic Medicine

Abstract

Antibacterial activity of biosynthesized silver nanoparticles (AgNPs) was significant in therapeutic application of nanotechnology. This research studied an ecofriendly and rapid method for the first time to synthesize silver nanoparticles using Zizyphus spina christi aqueous leaves extract (ZSE), and their antibacterial properties. The extract was found to have the potential to form silver nanoparticles at room temperature within few minutes. The nanoparticles were characterized using different techniques. The UV-visible spectrum showed a peak at 414 nm corresponding to the plasmon absorbance of silver nanoparticles. The transmission electron microscopy (TEM) showed that the formed particles were hexagonal in shape with appreciable Nano size ranging from 21.5 to 59.67 nm. Fourier Transform Infrared Spectroscopy analysis (FTIR) of biosynthesized AgNPs affirmed the role of ZSE as reducing and capping agent of Ag+ ions to AgNPs, and X-Ray Diffraction patterns (XRD) showed that they could be indexed as face-centered-cubic structure of silver. Antibacterial activity of AgNPs was determined by well diffusion and micro plate assay methods, showing inhibition zones of 24 mm, 23 mm, 15 mm and 17 mm against Staphylococcus aureus, Acinetobacter sp., Pseudomonas aeruginosa and Escherichia coli respectively. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) results showed that Ag NPs had MIC, MBC of 45, 57 μg/mL, 49, 61 μg/mL, 63, 90 μg/mL and 59, 82 μg/mL against tested pathogen respectively. Furthermore, the green synthesized AgNPs loaded on band-aids exhibited strong antibacterial effect against multi drug resistant bacteria. These nanoparticles could be used for treating wounds and preparing wound dressing. Such researches are crucial in the demonstration of therapeutic importance of silver nanoparticles as new alternative in medical application.